Evolutionary model of brain tumor circulating cells: Cellular galaxy

Figure 14  Protein expression of cytokeratin-19, CD45, and cyclin E in circulating tumor cells of a vasculature image of a breast-brain metastatic patient. Cytokeratin-19 (CK-19), CD45, and cyclin E are conjugated with phycoerythrin-indodicarbocyanine, R-phycoerythrin, and fluorescein isothiocyanate, respectively. A: 4′,6-Diamidino-2-phenylindole (DAPI)/CK-19 [highest/positive protein expression (PE)]; B: DAPI/CD45 (negative PE); C: DAPI/cyclin E (positive PE: Partial at left clone of cells). Harmonic PE between CK-19 and cyclin E, arrows indicate lack of cyclin E expression in the middle of vasculature in patient 17.