Evolutionary model of brain tumor circulating cells: Cellular galaxy

Figure 11  Expression mode of proteins of C-C chemokine ligand 2, vascular endothelial growth factor, and epidermal growth factor in circulating tumor cells from a tumor of patient affected with cerebellar medulloblastoma. A: Merged image of cells conjugated with 4′,6-diamidino-2-phenylindole (DAPI)/C-C chemokine ligand 2 (CCL2)/vascular endothelial growth factor (VEGF)/epidermal growth factor (EGF) is reflective of remarkable co-expression between VEGF/EGF proteins; B: Merged cells with DAPI/CCL2 indicating the absence of expression in the majority of cells; C: Merged cells with DAPI/VEGF with high expression; D: Merged cells with DAPI/EGF reflect lower expression than VEGF. Asterisks are referred to the cluster of CTCs.