Evolutionary model of brain tumor circulating cells: Cellular galaxy

Figure 10  Expression mode of C-C chemokine ligand 2, vascular endothelial growth factor and epidermal growth factor in the migrated vasculature into the blood stream in a patient affected with metastatic brain tumor with breast origin. C-C chemokine ligand 2 (CCL2), vascular endothelial growth factor (VEGF) and epidermal growth factor (EGF) are conjugated with fluorescein isothiocyanate, R-phycoerythrin, and phycoerythrin–indodicarbocyanine, respectively in patient 17. A: Merged of 4′,6-diamidino-2-phenylindole (DAPI)/CCL2/VEG/EGF; B, C and D: Partially cropped section between two asterisk of image A and present the merged of DAPI/CCL2, DAPI/VEGF and DAPI/EGF respectively; B and C: Indicates the involvement of traceable tumor cells with higher expression than EGF within a migrated vasculature to the blood stream; E, F and G: Present co-expression between CCL2/Veg, CCL2/EGF and EGF/VEGF respectively, there is more harmony between CCL2/Veg and CCL2/Veg.