Evolutionary model of brain tumor circulating cells: Cellular galaxy

Figure 9 Status of protein expression and co-expression of C-C chemokine ligand 2/vascular endothelial growth factor/epidermal growth factor through migration of circulating tumor cells within tumor territory to the blood stream in a patient with the lung metastatic carcinoma. C-C chemokine ligand 2 (CCL2), vascular endothelial growth factor (VEGF) and epidermal growth factor (EGF) are conjugated with fluorescein isothiocyanate; R-phycoerythrin, and phycoerythrin–indodicarbocyanine, respectively, in patient 15. A: Merged of 4′,6-diamidino-2-phenylindole (DAPI)/CCL2/EGF/VEGF; B: Cropped section of image a; C: Merged of DAPI/CCL2; D: Merged of DAPI/VEGF; E: Merged of DAPI/EGF; F: Co-expression of CCL2/Veg; G: Co-expression of CCL2/EGF; H: Co-expression of CCL2/VEGF/EGF. Merged of DAPI/CCL2/VEGF/EGF is reflective of co-expression between three proteins; B, F-H: Reflect co-expression of CCL2/VEGF/EGF according to the selected of cropped section from images a (arrow on the top/left). The arrows on the left side of images are referred to the migration of tumor cells from tumor to the blood stream destination through a bubble like channel.