Evolutionary model of brain tumor circulating cells: Cellular galaxy

Figure 8 Mode of protein expression and co-expression of C-C chemokine ligand 2, vascular endothelial growth factor, and epidermal growth factor of the circulating tumor cells of patient 13 with meningioma. C-C chemokine ligand 2 (CCL2), vascular endothelial growth factor (VEGF), and epidermal growth factor (EGF) are conjugated with fluorescein isothiocyanate, R-phycoerythrin, and phycoerythrin–indodicarbocyanine, respectively. A: Leukocytes and circulating tumor cells (CTCs) with 4′,6-diamidino-2-phenylindole; B: Image of the same cells with CCL2; C: Image of the same cells with VEGF; D: Image of the same cells with EGF; E: Co-expression of the CCL2/VEGF/EGF in the same cells; F and G: Co-expression of the CCL2/VEGF/EGF in the selected cropping cells (1 and 2) from A. The arrows are reflective of high protein co-expression within CTCs according the mode of expression in images b-d in the EGF; and the VEGF reflects the lowest expression.