Evolutionary model of brain tumor circulating cells: Cellular galaxy

Figure 5 Mode of protein expression and co-expression of C-C chemokine ligand 2, vascular endothelial growth factor, and epidermal growth factor in circulating tumor cells of a patient with meningioma. C-C chemokine ligand 2 (CCL2), vascular endothelial growth factor (VEGF) and epidermal growth factor (EGF) are conjugated with fluorescein isothiocyanate, R-phycoerythrin, and phycoerythrin–indodicarbocyanine, respectively, in patient number 1. A: Merged of 4′,6-diamidino-2-phenylindole/CCL2; B: Phase contrast; C: Same cells with CCL2; D: Cells with VEGF; E: Cells with EGF; F: Merged/cropped cells with VEGF/EGF; G: Merged of 4′,6-diamidino-2-phenylindole /CCL2; H: Merged cells with dai/VEGF; I: Merged cells with dai/EGF; J: Cropped/merged cells with CCL2/VEGF; K: Cropped/merged cells with CCL2/EGF; L: Cropped/merged cells with CCL2/VEGF/EGF.