Diacerein treatment prevents colitis-associated cancer in mice

Figure 5 Diacerein treatment does not induce apoptosis or modulate cell proliferation. A: Quantification of apoptosis by terminal deoxynucleotidyl transferase-mediated dUTP nick end labelling and representative microphotographs of stained colon sections containing tumors of control (CTL) and diacerein (DAR) 50-treated mice (magnification 40 ×), scale bar: 50 µm; n = 5; five fields per colonic tissue section; mean ± standard error; B: Quantification of Ki-67⁺ and representative microphotographs of stained colon sections containing tumors of CTL and DAR50-treated mice (magnification 40 ×), scale bar: 50 µm, n = 5; five fields per colonic tissue section; mean ± standard error; C: Analysis of protein expression involved in tumor growth signaling pathways (p-AKT, p-70S6K) by immunoblot analysis in colons and colonic tumors of Swiss mice (CTL and DAR50) (Supplementary Figures 3 and 4). Data are presented as mean ± standard error.