Mutational analysis of Ras hotspots in patients with urothelial carcinoma of the bladder

Table 3 Primer sequences used in direct DNA Sequencing

Gene	Strand	Coding exon	Primer sequences	Length of amplified fragment
K-Ras	+	1	F 5'-TTAACCTTATGTGTGACATGTTCTAA-3'	378 bp
K-Ras	-	1	R 5'-CCCTGACATACTCCCAAGGA-3'
K-Ras	+	2	F 5'- TCAAGTCCTTTGCCCATTTT-3'	375 bp
K-Ras	-	2	R 5'- TGCATGGCATTAGCAAAGAC-3'
N-Ras	+	1	F 5'-GCCCAAGGACTGTTGAAAAA-3'	477 bp
N-Ras	-	1	R 5'-TGCATAACTGAATGTATACCCAAAA-3'
N-Ras	+	2	F 5'-GGCAGAAATGGGCTTGAATA-3'	424 bp
N-Ras	-	2	R 5'-CCTAAAACCAACTCTTCCCATAA-3'
H-Ras	+	1	F 5'-GTGGGTTTGCCCTTCAGAT-3'	386 bp
H-Ras	-	1	R 5'-TCTAGAGGAAGCAGGAGACAGG-3'
H-Ras	+	2	F 5'-CAGGACACAGCCAGGATAGG-3'	492 bp
H-Ras	-	2	R 5'-ACATGCGCAGAGAGGACAG-3'

F: Forward strand (+); R: Reverse strand (-); bp: Base pair.