Mutational analysis of Ras hotspots in patients with urothelial carcinoma of the bladder

Table 2 Summary of Ras gene assays

Gene	Target codon	Restriction enzyme/site	Fragment size
			Undigested	Mutant after digestion	Wild-type/normal after digestion
K-Ras	12 Glycine (GGT) to Valine (GTT)/Aspartic acid (GAT)/Serine (AGT)/Arginine (CGT)/Alanine (GCT)/Cysteine (TGT)	MvaI (BstNI)	144 bp	144 bp	115 bp and 29 bp
		CC↓ WGG
		GGW↑ CC
K-Ras	13 Glycine (GGC) to Aspartic acid (GAC)	HphI	144 bp	101 bp and 43 bp	144 bp
		GGTGAN8↓
		CCACTN7↑
H-Ras	12 Glycine (GGC) to Valine (GTC)/Aspartic acid (GAC)/Serine (AGC)/Alanine (GCC)	Mspl (HpaII)	420 bp	420 bp	390 bp and 30 bp
		(C↓ CGG)
		(GGC↑ C)
N-Ras	61 Glutamine (CAA) to Arginine (CGA)/Lysine (AAA)/Leucine (CTA)	Mlsl (MscI)	65 bp	65bp	44 bp and 21 bp
		(TGG↓ CCA)
		(ACC↑ GGT)

bp: Base pair.