Mutational analysis of Ras hotspots in patients with urothelial carcinoma of the bladder

Table 1 Primer sequences used for polymerase chain reaction - restriction fragment length polymorphism

Gene	Target codon	Strand	Primer sequences
H-Ras	12	+	5’GACGGAATATAAGCTGGTGG 3’
		-	5’AGGCACGTCTCCCCATCAAT 3’
K-Ras	12 and 13	+	5’ACTGAATATAAACTTGTGGTAGTTGGACCT 3’
		-	5’TTCTCCATCAATTACTACTTGCTTCCTGTA 3’
N-Ras	61	+	5’GACATACTGGATACAGCTGGC 3’
		-	5’CCTGTCCTGATGTATTGGTC 3’

+: Forward strand; -: Reverse strand.