Forkhead box P3 and indoleamine 2,3-dioxygenase co-expression in Pakistani triple negative breast cancer patients

Figure 1 Formalin-fixed paraffin-embedded tumor specimens. A and B: Forkhead box P3 (FOXP3) immunohistochemical staining; A: Invasive ductal carcinoma with FOXP3-negative expression; B: FOXP3-positive lymphocytic infiltration in invasive ductal carcinoma. The staining was nuclear; C and D: Co-expression of FOXP3 and indoleamine 2,3-dioxygenase (IDO), FOXP3 and IDO expression in breast cancer tissues (n = 100) were evaluated; C: FOXP3 positive cells infiltrated in invasive ductal carcinoma (nuclear staining). Sections from matched breast cancer patients were stained for IDO; D: Strong and diffuse IDO staining in invasive ductal tumor cells (cytoplasmic staining). Images were captured at × 40 magnification.