Tumor-specific lytic path “hyperploid progression mediated death”: Resolving side effects through targeting retinoblastoma or p53 mutant

Figure 3 Nocodazole triggers hyperploid progression mediated death in retinoblastoma-mutant WERI-1 retinoblastoma cells. A DNA content flow cytometric histogram of WERI-1 cells continuously treated with nocodazole (Noc; 0.415 µmol/L) for the duration (1 d, 24 h; 4 d, 96 h) is shown. Cells (5 × 10⁵) were treated as indicated, harvested using trypsin and fixed in 70% ethanol. To determine DNA content per cell, propidium (100 Kunitz units/mL) and RNase A (50 µg/mL) were added and assayed using Beckton Dickinson flow cytometry. Persistent treatment with nocodazole led to the death of hyperploid cells induced. RB: Retinoblastoma.