Updates on “Cancer Genomics and Epigenomics”

Table 2 Circulating tumor cell isolation techniques

Technology	Platform	Methods	Ref.
Physical properties	Density gradient, physical filter, dielectric, photoacoustic microfluidic	Size, density	[35,36]
High-throughput imaging	Imaging cytometry	Scanning of cells on the slide	[36]
Leukocyte depletion	Batch cell lysis; microfluidic CTC-iChip; immunomagnetic separation	Negative depletion of leukocytes	[37]
Antibody capture	Cell search; magsweeper; microfluidic CTC-chip	Selection for tumor-specific markers	[37]
Functional characteristics	Epispot assay, invasion assay	Protein secretion, cell migration	[38]
Nanotechnology	Immunomagnetic nanobeads, nano-structures substrates in microchip	Nanomaterials able to increase interactions with CTCs and specific antibodies, and to enable their electrical conductivity	[39]

CTC: Circulating tumor cell.