DNA extraction from paraffin embedded colorectal carcinoma samples: A comparison study of manual vs automated methods, using four commercially kits

Table 6 Correlation between DNA concentration¹ and clinicopathological aspects of colorectal cancer

Characteristics		Number	High Pure FFPE DNA isolation kit (manual)		P value
			< 100 ng/µL	≥ 100 ng/µL
Age	≤ 60	20	12	8	0.993
	> 60	26	15	11
Gender	Male	26	15	11	0.993
	Female	20	12	8
Macroscopic aspect	Polypoid	10	8	17	0.123
	Ulcero-infiltrative	36	19	2
Microscopic aspect	G1	13	3	10	0.0023
	G2 + G3	33	24	9
Localization	Proximal colon	8	5	3	0.962
	Distal colon	21	12	9
	Rectum	17	10	7
Depth of infiltration (pT stage)	T2-T3 T4	40 6	24 3	16 3	0.642
Lymph node metastasis (pN stage)	Present (pN1-3)	17	8	9	0.353
	Absent (pN0)	29	19	10
Lymph node ratio	< 0.15	36	23	13	0.273
	≥ 0.15	10	4	6

¹Manual High Pure FFPE DNA isolation kit-Roche.

²Chi square test.

³Fisher’s exact test. G1: Well differentiated; G2: Moderately differentiated; G3: Poorly differentiated.