DNA extraction from paraffin embedded colorectal carcinoma samples: A comparison study of manual vs automated methods, using four commercially kits

Table 4 Correlation between DNA concentration¹ and clinicopathological aspects of colorectal cancer

Characteristics		Number	DNA concentration PureLink Genomic DNA mini kit (manual)		P value
			≤ 150 ng/µL	> 150 ng/µL
Age	≤ 60	20	12	8	0.373
	> 60	26	11	15
Gender	Male	26	15	11	0.373
	Female	20	8	12
Macroscopic aspect	Polypoid	10	4	15	0.00103
	Ulcero-infiltrative	36	19	8
Microscopic aspect	G1	13	3	10	0.043
	G2 + G3	33	20	13
Localization	Proximal colon	8	5	3	0.892
	Distal colon	21	11	10
	Rectum	17	7	10
Depth of infiltration (pT stage)	T2-T3 T4	40 6	20 3	20 3	> 0.992
Lymph node metastasis (pN stage)	Present (pN1-3)	17	13	4	0.063
	Absent (pN0)	29	10	19
Lymph node ratio	< 0.15	36	17	19	0.723
	≥ 0.15	10	6	4

¹Manual PureLink Genomic DNA mini kit-Invitrogen.

²Chi square test.

³Fisher’s exact test. G1: Well differentiated; G2: Moderately differentiated; G3: Poorly differentiated.