Basic Study
Copyright ©The Author(s) 2019.
World J Clin Oncol. Jun 24, 2019; 10(6): 222-233
Published online Jun 24, 2019. doi: 10.5306/wjco.v10.i6.222
Figure 4
Figure 4 Leptin induces cell cycle progression and proliferation of endometrial cancer cells. A: Leptin induces S-phase progression of endometrial cancer (EmCa) cells. Cells were cultured with leptin (0 and 1.2nM) for 24 h. Cell cycle progression was determined by image cytometry using a Cellometer Vision CBA system (Nexcelom Biosciences, Lawrence, MA); B: Results of MTT assay from EmCa cells (type I: Ishikawa and HEC1A, and type II: An3Ca and KLE) cultured with leptin (0, 0.6, 1.2 and 6.2 nM) for 24 h. Absorbance was determined at 540 nm, and data was evaluated with Spectramax software. Data (mean ± SE) are representative of the results derived from a minimum of three independent experiments. gP < 0.05 and hP < 0.01 vs basal (no leptin).