Leptin-induced Notch and IL-1 signaling crosstalk in endometrial adenocarcinoma is associated with invasiveness and chemoresistance

Figure 2 Dose-response effects of leptin on the levels of Notch mRNA in endometrial cancer cells. A, B: Notch mRNA levels (receptors: Notch1, Notch2, Notch3 and Notch4; ligands: JAG1 and DLL4 and targets: Survivin, Hey2) induced by leptin (0, 0.6, 1.2 and 6.2nM) in type I endometrial cancer (EmCa) (A, Ishikawa) and type II EmCa cells (B, An3Ca). Cells were cultured for 24h. Notch mRNA levels were quantified by real-time RT-PCR. Cells were cultured for 24h. RNA expression was calculated by normalizing values to GAPDH mRNA. Data (mean ± SE) representative results derived from a minimum of 3 independent experiments. ^cP < 0.05 and ^dP < 0.01 vs basal (no leptin).