Plecanatide-mediated activation of guanylate cyclase-C suppresses inflammation-induced colorectal carcinogenesis in Apc+/Min-FCCC mice

Figure 7 Effect of plecanatide on cytokine expression. Expression of cytokines, chemokines and growth factors was measured in the spent media of colonic explant cultures derived from mice treated with DSS only or DSS + plecanatide. Murine cytokines, chemokines and growth factors were measured in pooled supernatants (n = 6/pool) using membranes coated with specific capture antibodies (Proteome Profiler Panel A kit; R and D Systems, Minneapolis, MN). Immunoblots were scanned using Image J software. The data represent the mean intensity of cytokine/chemokine levels in colonic samples derived from untreated and plecanatide-treated mice. Student’s t test was used to evaluate statistical differences in the secretion of cytokines/chemokines between DSS control and DSS + plecanatide treated samples. ^aP value ≤ 0.05, ^bP≤ 0.01; vs DSS control.