A20 inhibits lipopolysaccharide-induced inflammation in enterocytes

Figure 5 A20 inhibited nuclear factor-κB activation and translocation to the nucleus. Immunofluorescence was performed to detect the intracellular localization of NF-κB using anti-NF-κB p65 primary antibodies and fluorescent secondary antibodies (red) followed by confocal microscopy. DAPI-stained nuclei appear in blue. Weaker fluorescence intensity in the nucleus was detected in A20 overexpression cell lines.