A20 inhibits lipopolysaccharide-induced inflammation in enterocytes

Figure 1 Expression of A20 in different groups. HT-29 cells were transduced with LV-A20, LV-shA20, or LV-controls. Cytosolic lysates were tested by Western blotting using anti-A20 antibodies or anti-β-actin antibodies to normalize protein levels. In A20 overexpression cell lines, the expression of A20 was much higher than that of the control. Two shRNA sequences, A and B, were obtained, and the expression of A20 in both of the A20 knockdown groups were much lower than the control. The effect of A was more pronounced than B. Thus, sequence A was selected for subsequent experiments.