Effects of aging on the architecture of the ileocecal junction in rats

Figure 3 Immunohistochemistry mistry final. HuC/D staining in immunoreactive neurons (A, D), NOS staining in immunoreactive neurons and nerve fibers (B, E), and DAPI staining of the nuclei of immunoreactive cells (C, F) of the ileocecal junction in young rats (A-C) and elderly rats. The co-localization of HuC/D, NOS and DAPI (C, F). The arrows indicate immunoreactive neurons stained for HuC/D (A, D), NOS (B, E), and triple colocalization with HuC/D, NOS and DAPI (C, F). The double arrows indicate immunoreactive fibers stained for NOS (B, E). The double arrows show muscle fiber nuclei stained with DAPI (C, F). NOS: Nitric oxide synthage; DAPI: 4',6-diamidino-2-phenylindole.