Oral tolerance is inducible during active dextran sulfate sodium-induced colitis

Figure 5 Evaluation of cytokine mRNA expression in the spleen and mesenteric lymph nodes during colitis. A: Evaluation of cytokine mRNA expression in the spleen during colitis. IFN-γ mRNA expression was significantly lower in the spleens of mice with colitis compared to those of mice without colitis. There were no significant differences in IL-4, IL-6, IL-10 or TNF-α mRNA expression; B: Evaluation of cytokine mRNA expression in the MLN in mice with colitis. In the MLN, there was no significant difference in IL-4, IL-6, IL-10, IFN-γ, or TNF-α expression between mice with and without colitis. Expression values were normalized to the expression of the housekeeping gene GAPDH. All results represent at least two independent experiments with four to six mice in each group. The data are shown as the median and interquartile range. ^bP < 0.01. MLN: Mesenteric lymph nodes; DSS: Dextran sulfate sodium; TNF: Tumor necrosis factor; IFN: Interferon; IL: Interleukin.