Oral tolerance is inducible during active dextran sulfate sodium-induced colitis

Figure 3 Regulatory T cell frequencies in the spleen and mesenteric lymph nodes as determined by flow cytometry. A: Analysis of CD25 and Foxp3 expression in the spleen. Representative data are shown for control mice (left panel) and DSS colitis mice (right panel); B: CD4⁺CD25⁺ cell frequencies in the spleens of DSS colitis mice and control mice. CD4⁺CD25⁺ cell frequency tended to increase in the spleens of DSS colitis mice (closed bar) compared to those of control mice (open bar); C: CD4⁺Foxp3⁺ cell frequencies in the spleens of DSS colitis mice and control mice. CD4⁺Foxp3⁺ cell frequency was comparable in the spleens of DSS colitis mice (closed bar) and control mice (open bar); D: CD4⁺CD25⁺Foxp3⁺ cell frequencies in the spleens of DSS colitis mice and control mice. CD4⁺CD25⁺Foxp3⁺ cell frequency was comparable in the spleens of DSS colitis mice (closed bar) and control mice (open bar); E: CD4⁺CD25^-Foxp3⁺ cell frequencies in the spleens of DSS colitis mice and control mice. CD4⁺CD25^-Foxp3⁺ cell frequency was comparable in the spleens of DSS colitis mice (closed bar) and control mice (open bar); F: Analysis of CD25 and Foxp3 expression in the MLN. Representative data are shown for control mice (left panel) and DSS colitis mice (right panel); G: CD4⁺CD25⁺ cell frequencies in the MLNs of DSS colitis and control mice. CD4⁺CD25⁺ cell frequency was comparable in the MLNs of DSS colitis mice (closed bar) and control mice (open bar); H: CD4⁺Foxp3⁺ cell frequencies in the MLNs of DSS colitis mice and control mice. CD4⁺Foxp3⁺ cell frequency tended to increase in the MLNs of DSS colitis mice (closed bar) compared with control mice (open bar); I: CD4⁺CD25⁺Foxp3⁺ cell frequencies in the MLNs of DSS colitis mice and control mice. CD4⁺CD25⁺Foxp3⁺ cell frequency was comparable in the MLNs of DSS colitis mice (closed bar) and control mice (open bar); J: CD4⁺CD25^-Foxp3⁺ cell frequencies in the MLNs of DSS colitis mice and control mice. CD4⁺CD25^-Foxp3⁺ cell frequency was significantly higher in the MLNs of DSS colitis mice (closed bar) compared with control mice (open bar). All results represent at least two independent experiments with four to six mice in each group. The data are shown as the median and interquartile range. ^aP < 0.05. MLN: Mesenteric lymph nodes; DSS: Dextran sulfate sodium.