Nociceptin effect on intestinal motility depends on opioid-receptor like-1 receptors and nitric oxide synthase co-localization

Figure 4 Double-labeling of myenteric plexus whole-mount preparations with nociception, nitric oxide synthase and neuron-endocrine marker protein gene product 9. 5 immunoreactivity. A: Double-labeling of myenteric plexus whole-mount preparations of mouse colon demonstrating co-labeling in myenteric neurons of nociceptin (NOC) immunoreactivity with nitric oxide synthase (NOS) (A-C) and the neuron-endocrine marker protein gene product 9.5 (PGP9.5) (D-F); B: A granular appearance of the nociceptin staining in the myenteric neurons was also observed in brain sections, which served as additional controls. Individual immunopositive neurons are recognizable along the entire cortex, be it with slight region-dependent differences in staining intensity. In the hippocampal region also neuronal processes stain for nociceptin. The diffuse distribution of nociceptin corresponds with literature data (see also datasheet Abcam). No other structures apart from neurons appear to be stained.