Nociceptin effect on intestinal motility depends on opioid-receptor like-1 receptors and nitric oxide synthase co-localization

Figure 2 A representative gel for reverse transcriptase - polymerase chain reaction for opioid-receptor like-1 using total RNA. Two alternatively spliced mRNA variants of mouse opioid-receptor like-1 (ORL1) with a size of approximately 320 and 400 bp are expressed in mouse brain (lane 1), longitudinal muscle-myenteric plexus layer (LMMP) (lane 2) and mucosa (lane 3) of mouse ileum and in LMMP (lane 4) of mouse colon. In the mucosa of mouse colon, only the short ORL1 isoform is present (lane 5), whereas the negative control (lane 6) showed no specific polymerase chain reaction product. Lower panel shows results for GAPDH, which was used as internal control. GAPDH: Glyceraldehyde 3-phosphate dehydrogenase.