Copyright
©The Author(s) 2015.
World J Gastrointest Pharmacol Ther. Aug 6, 2015; 6(3): 73-83
Published online Aug 6, 2015. doi: 10.4292/wjgpt.v6.i3.73
Published online Aug 6, 2015. doi: 10.4292/wjgpt.v6.i3.73
Table 1 Antisera used for immunohistochemistry
| Antisera (host) | Source | Dilution |
| Primary antisera | ||
| ORL1 (KOR-3) (H-85) (rabbit) | Santa Cruz Biotechnology (sc-15309) | 1:50 |
| ORL1 (KOR-3) (N-19) (goat) | Santa Cruz Biotechnology (sc-9759) | 1:50 |
| Nociceptin (guinea-pig) | Abcam (ab10276) | 1:100-200 |
| NOS (rabbit) | Euro Diagnostica (B 220-1) | 0.389 |
| NOS (guinea pig) | Euro Diagnostica (B-GP 225-1) | 0.736 |
| PGP 9.5 (guinea pig) | Chemicon (AB5898) | 0.389 |
| Secondary antisera and streptavidin complexes | ||
| FITC-conjugated donkey anti rabbit | Jackson ImmunoResearch (711-095-152) | 0.111 |
| Cy3-conjugated donkey anti rabbit | Jackson ImmunoResearch (711-165-152) | 0.215 |
| Biotinylated donkey anti rabbit | Jackson ImmunoResearch (711-065-152) | 0.111 |
| Biotinylated donkey anti goat | Jackson ImmunoResearch (705-065-147) | 0.111 |
| Biotinylated donkey anti goat | Jackson ImmunoResearch (706-065-148) | 0.111 |
| FITC-conjugated donkey anti goat | Jackson ImmunoResearch (705-095-147) | 1:50 |
| FITC-conjugated donkey anti guinea pig | Jackson ImmunoResearch (706-095-148) | 1:50 |
| Cy3-conjugated donkey anti guinea pig | Jackson ImmunoResearch (706-165-148) | 0.215 |
| Cy3-conjugated Streptavidin | Jackson ImmunoResearch (016-160-084) | 0.215 |
| FITC-conjugated Streptavidin | Jackson ImmunoResearch (016-010-084) | 1:100 Streptavidin FITC: Jackson ImmunoResearch 016-010-084; 1:100 |
- Citation: Sibaev A, Fichna J, Saur D, Yuece B, Timmermans JP, Storr M. Nociceptin effect on intestinal motility depends on opioid-receptor like-1 receptors and nitric oxide synthase co-localization. World J Gastrointest Pharmacol Ther 2015; 6(3): 73-83
- URL: https://www.wjgnet.com/2150-5349/full/v6/i3/73.htm
- DOI: https://dx.doi.org/10.4292/wjgpt.v6.i3.73