Bowel adhesion and therapy with the stable gastric pentadecapeptide BPC 157, L-NAME and L-arginine in rats

Figure 8 Support was obtained from the presentation of MDA- and NO tissue levels in adhesion tissues. A: At 7 d post-injury, we determined nitric oxide (NO) in adhesion tissue samples using the Griess reaction. Medication (/kg, 10 mL/2 min bath/rat) at abdominal cavity was BPC 157 (10 µg) (B), L-arginine (A) (100 mg), L-NAME (5 mg) alone or in combinations L-NAME + L-arginine (NA), L-arginine + BPC 157 (AB), L-NAME + BPC 157 (NB), L-arginine + L-NAME + BPC 157 (ANB) or saline bath equal volume (controls) (C). ^aP < 0.05 at least vs control; B: At 14 d post-injury, we determined NO in adhesion tissue samples using the Griess reaction. Medication (/kg, 10 mL/2 min bath/rat) at abdominal cavity was BPC 157 (10 µg) (B), L-arginine (A) (100 mg), L-NAME (5 mg) alone or in combinations L-NAME + L-arginine (NA), L-arginine + BPC 157 (AB), L-NAME + BPC 157 (NB), L-arginine + L-NAME + BPC 157 (ANB) or saline bath equal volume (controls) (C). ^aP < 0.05 at least vs control; C: At 7 d post-injury, we determined oxidative stress in adhesion tissue samples was assessed by quantifying thiobarbituric acid reactivity as malondialdehyde equivalents. Medication (/kg, 1 mL bath/rat) at abdominal cavity was BPC 157 (10 µg) (B), L-arginine (A) (100 mg), L-NAME (5 mg) alone or in combinations L-NAME + L-arginine (NA), L-arginine + BPC 157 (AB), L-NAME + BPC 157 (NB), L-arginine + L-NAME + BPC 157 (ANB) or saline bath equal volume (controls) (C). ^aP < 0.05 at least vs control; and D: At 14 d post-injury, we determined oxidative stress in adhesion tissue samples was assessed by quantifying thiobarbituric acid reactivity as malondialdehyde equivalents. Medication (/kg, 1 bath/rat) at abdominal cavity was BPC 157 (10 µg) (B), L-arginine (A) (100 mg), L-NAME (5 mg) alone or in combinations L-NAME + L-arginine (NA), L-arginine + BPC 157 (AB), L-NAME + BPC 157 (NB), L-arginine + L-NAME + BPC 157 (ANB) or saline bath equal volume (controls)(C). ^aP < 0.05 at least vs control.