Pharmacological inhibition of diacylglycerol acyltransferase-1 and insights into postprandial gut peptide secretion

Figure 5 Incretin effect suppressed postprandially over time with pharmacological diacylglycerol acyltransferase-1 inhibition in GPR119 -/- mice. Fasted male GPR119 -/- and +/+ mice were administered vehicle or 10 mg/kg of PF-04620110 by oral gavage (n = 8 per group). Thirty minutes post compound administration mice received a bolus of corn oil (challenge). Blood was collected just prior to bolus (time 0) and at 1, 2, 4 and 6 h afterwards for determination of active/total GLP-1, PYY and GIP concentrations. (A) Active GLP-1 (pmol/L), (C) total GLP-1, (E) total PYY (pg/mL) and (G) total GIP (pg/mL) in C57BL/6J mice treated with vehicle or PF-04620110 following challenge. AUC values for (B) active GLP-1, (D) total GLP-1, (F) total PYY and (H) total GIP. Treatments compared to vehicle +/+ (^aP < 0.05, ^bP < 0.01, ^dP < 0.001 ANOVA). Values are means ± SEM. DGAT: Diacylglycerol acyltransferase-1; GLP-1: glucagon like peptide-1.