Multiple endocrine neoplasia 2B: Differential increase in enteric nerve subgroups in muscle and mucosa

Figure 4 NOS, VIP and SP nerve fibres in myenteric and submucosal ganglia and circular muscle in normal colon and MEN2B colon. A: NOS-; B: VIP-; C: SP-immunoreactivity in Control (HSCR) patient; D: NOS-; E: VIP-; F: SP-immunoreactivity in MEN2B patient. Note all images are at the same magnification. The enlarged myenteric ganglia (MG) contain many NOS, VIP and SP nerve fibres with NOS at the highest density. The submucosal (SM) ganglia also contain all 3 labels, but SP is most intense and extremely bright in MEN2B. In the circular muscle (CM), NOS is the most abundant and highly increased in MEN2B. Full thickness biopsy of the transverse colon cut with circular muscle in cross section.