Multiple endocrine neoplasia 2B: Differential increase in enteric nerve subgroups in muscle and mucosa

doi:10.4291/wjgp.v8.i3.142

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World Journal of Gastrointestinal Pathophysiology

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2150-5330

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Baishideng Publishing Group Inc, 7041 Koll Center Parkway, Suite 160, Pleasanton, CA 94566, USA

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World J Gastrointest Pathophysiol. Aug 15, 2017; 8(3): 142-149
Published online Aug 15, 2017. doi: 10.4291/wjgp.v8.i3.142

Table 1 Antibodies used in the study

Molecule	Company	Dilution
Primary antibody
Goat anti c ChAT	AB5966 Chemicon International, Temecula, CA, United States	1:100
Rabbit anti nNOS	AB 5380, Chemicon International, Temecula, CA, United States	1:1000
Rabbit anti VIP	NCL VIPp, Novocastra Laboratories, Newcastle-upon-Tyne, United Kingdom	1:200
Rabbit anti SP	18-0091, Zymed Laboratories, South San Francisco, CA, United States	1:50
Goat anti CGRP	1720-9-7, AbD Serotec, Biorad	1:1000
Rabbit anti-human cKit	Polyclonal CD117, Dako	1:200
Secondary antibody
Goat anti rabbit Alexa 488	Molecular Probes, ThermoFisher Scientific A-11012	1:400
Donkey anti rabbit Alexa 488	Molecular Probes, ThermoFisher Scientific, A-21206	1:200
Goat anti mouse Alexa 488	Molecular Probes, ThermoFisher Scientific, A11001	1:400
Donkey anti goat Alexa 568	Molecular Probes, ThermoFisher Scientific, A11057	1:400

Citation: Hutson JM, Farmer PJ, Peck CJ, Chow CW, Southwell BR. Multiple endocrine neoplasia 2B: Differential increase in enteric nerve subgroups in muscle and mucosa. World J Gastrointest Pathophysiol 2017; 8(3): 142-149
URL: https://www.wjgnet.com/2150-5330/full/v8/i3/142.htm
DOI: https://dx.doi.org/10.4291/wjgp.v8.i3.142