Combination curcumin and vitamin E treatment attenuates diet-induced steatosis in Hfe-/- mice

Figure 4 Curcumin and vitamin E treatments increase hepatic antioxidant enzyme activities and increase mitochondrial biogenesis. Hepatic tissue from Hfe-/- mice fed HCD for 10 wk then HCD + Cu, HCD + VE or HCD + Cu + VE for a further 10 wk (n = 7-9) was analysed for (A) glutathione peroxidase (GPx) activity, catalase activity and mitochondrial superoxide dismutase (MnSOD) activity. qRT-PCR was used to determine expression levels of hepatic (B) nuclear transcription factor 1 (Nrf-1) and transcription factor B2 mitochondrial (TfB2M). C: Western blotting and densitometry analysis was performed to determine levels of hepatic mitoNEET and glyceraldehyde 3-phosphate dehydrogenase (Gapdh). Data are depicted using box and whisker plots showing median, minimum and maximum values. Statistical significance was tested using Kruskal-Wallis test with Dunn’s correction for multiple comparisons, ^aP ≤ 0.05, ^bP ≤ 0.01, ^eP ≤ 0.001. HCD: High calorie diet; Cu: Curcumin; VE: Vitamin E.