Neurophysiological mechanisms of bradykinin-evoked mucosal chloride secretion in guinea pig small intestine

Figure 6 Post-receptor signal transduction for bradykinin-evoked increase in baseline increase in short-circuit current. The BK-evoked Isc was suppressed in the presence of the PLC inhibitor U73122, the PKC inhibitor Bis-I, the calmodulin inhibitor W7, the calmdulin kinase II inhibitor KN-62 (10 μM), or the MAPK pathway inhibitor PD 98059 (^eP < 0.01 vs BK alone). Inhibitor of PKA (KT5720 1 μM) or inhibitor of tyrosine protein kinase (genistein 10 μM) did not have a significant effect on BK-evoked increase in Isc. Isc: Increase in short-circuit current; BK: Bradykinin; PLC: Phospholipase C; PKC: Protein kinase C; Bis-I: Bisindolymaleimide I.