Oxidative and nitrosative stress enzymes in relation to nitrotyrosine in Helicobacter pylori-infected humans

Figure 2 Western blot. A: Western blot detecting inducible nitric oxide syntase (iNOS) with a band at 130 kDa in RAW 264.7 (pos contr.), and in human antral mucosa retrieved from Helicobacter pylori (H. pylori) pos. and H. pylori neg. volunteers during endoscopy; B: Western blot of the MPO positive 60 kDa band in the positive HL60 control and in gastric mucosal specimens of H. pylori pos. and H. pylori neg. volunteers; C: Western blot of p47-phox, representing NADPH-oxidase with a band at 47 kDa (pos. contr.) HL60, and in HP+ and HP- samples; D: Regarding Western blot representing Nitrotyrosine, several bands of Nitrated proteins could be analysed. Shown in the figure is a typical 66 kDa band in the pos. control and in H. pylori pos. and H. pylori neg. subjects.