Prognostic significance of tumor budding, desmoplastic reaction, and lymphocytic infiltration in patients with gastric adenocarcinoma

Figure 1 Tumor budding grades assessed according to International Tumor Budding Consensus Conference recommendations, desmoplastic reaction, and tumor-infiltrating lymphocytes. A: TB1 (1-4 tumor bud/hot spot), 200 ×; B: TB2 (5-9 tumor bud/hot spot), 200 ×; C: TB3 (10 tumor bud/hot spot), 200 ×; D: Desmoplastic reaction 1 (DR1), mature stroma composed of tightly packed collagen fibers, 400 ×; E: DR2, intermediate stroma, consisting of areas of collagen that resemble keloids, 400 ×; F: DR3, immature stroma with myxoid alterations; G: Gastric carcinoma with high TILs in tumor stroma (≥ 5%), 100 ×; H: Gastric carcinoma with low TILs (< 5 %), 100 ×. Hematoxylin-eosin, black arrows indicate tumor buds.