Novel, non-colonizing, single-strain live biotherapeutic product ADS024 protects against Clostridioides difficile infection challenge in vivo

Figure 3 Distal colon model. A: Comparison of the wells inoculated with ADS024 vs media alone was done via quantitative polymerase chain reaction (qPCR) using specific primers to detect ADS024 as described in Materials and Methods; B: qPCR was performed to study the antimicrobial activity of ADS024 on 5 different phyla of the human colonic microbiota; C: MiSeq compositional sequencing analysis was performed to compare the impact of ADS024 on the gut microbiota at the phylum level with that of media alone; D: MiSeq was used to determine the ADS024 effect after 24 h of treatment at the genus level. ^eP ≤ 0.001.