Evaluating the regulation of transporter proteins and P-glycoprotein in rats with cholestasis and its implication for digoxin clearance

Figure 5 Effect of bile duct ligation on mRNA expressions of multidrug resistance 1, organic anion transporting polypeptides1a4 and 4C1. mRNA expression in each sample was standardized to its glyceraldehyde-3-phosphate dehydrogenase level. A: Expressions of multidrug resistance 1 in the liver, kidney, small intestine, and the effect of bile duct ligation (BDL) on the mRNA expressions in each tissue; B: Expression of organic anion transporting polypeptides (OATP)1A4 mRNA in the liver, kidney and small intestine, and the effect of BDL on OATP1A4 mRNA expressions; C: Expression of OATP4C1 mRNA in the kidney and the effect of BDL on its expression. Values are depicted as means ± SD; n = 6; ^aP < 0.05 compared with sham surgery rats. BDL: Bile duct ligation; OATP: Organic anion transporting polypeptides; MDR1: Multidrug resistance 1; GAPDH: Glyceraldehyde-3-phosphate dehydrogenase.