Chemokine receptor 8 expression may be linked to disease severity and elevated interleukin 6 secretion in acute pancreatitis

Figure 5 Representative moderately severe acute pancreatitis profile of the monocyte subpopulation. Cells were gated into intermediate (CD14+CD16+), classical (CD14+CD16-), non-classical monocytes (CD16+CD14-) and HLA-DR+/- monocytes. A and B: They showed CD14 PerCP Cy5.5 and CD16 PECy5 plot for Day 3 and Day 5 respectively. Classical monocyte subpopulations (CD14+CD16-) were higher in more severe patients with the MSAP patient having as much as 70% of the parent population on Day 3, which subsequently dropped to undetectable levels on Day 5. C and D: They showed a plot of CD14 PerCP Cy5.5 and HLA-DR BV650 for Day 3 and 5 respectively. The percentage of HLA-DR+ monocytes increased from 4% on Day 3 to 47% on Day 5. BUV: BD Horizon Brilliant™ Ultraviolet; Cy: Cyanine; BV: Brilliant Violet™; HLA DR: Human leukocyte D related; PE: Phycoerythrin; PerCP: Peridinin-Chlorophyll-protein; CD: Cluster of differentiation.