Comparison of cytokine and phosphoprotein profiles in idiopathic and Crohn’s disease-related perianal fistula

Figure 2 Boxplots for IL-12p70 concentration (left) and IL-1RA/IL-1β concentration ratios (right) in the supernatants from 24-h fresh tissue cultures using a 30-plex Milliplex MAP Human Cytokine/Chemokine Magnetic Bead Panel (EMD Millipore, Billerica, MA, United States) and a MAGPIX multiplexing instrument (Luminex Corporation, Austin, TX, United States). At the internal opening, differences between the idiopathic and Crohn’s disease groups were statistically significant. Median difference in IL-12p70 concentration at the internal opening was 19.7 pg/mL (Hodges-Lehman, 99%CI: 0.2-40.4; Mann-Whitney U, P = 0.008). The median difference in IL-1RA/IL-1β at the internal opening was 15.0 (Hodges-Lehman, 99%CI: 0.4-50.5; Mann-Whitney U, P = 0.008). Circle marker, outlier within 1.5 × interquartile range (IQR); Star marker, outlier out with 1.5 × IQR. One outlier not shown to limit Y-axis scale.