Estimation of intra-operator variability in perfusion parameter measurements using DCE-US

Figure 5 In vivo. A: Experiments were conducted with nude female mice aged from 6 to 8 wk. The selected tumor model was the B16F10 melanoma cell line which is a murine skin cancer; B, C: The region of interest contained only the tumor and was drawn for each injection performed. If the image was not well defined using the harmonic mode, the B-mode image was used to correctly define the region of interest; D: As in the case of the in vitro experiments, the ultrasound scanner displayed the associated time intensity curve. This was extracted and converted through an Excel^® file so that the analysis could be performed to obtain the required perfusion parameters.