Interleukin-19 is cardioprotective in dominant negative cyclic adenosine monophosphate response-element binding protein-mediated heart failure in a sex-specific manner

Figure 5 Expression of interleukin-20Rα/β and activation of signal transducer and activator of transcription 3 in male and female double transgenic mice. A: IL-20Rα and IL-20Rβ expression were similar in male and female DTG mice; B: Similarly, the ratio of IL-20Rα/β did not differ between sexes; C: STAT3 activation did not differ between male and female DTG mice; D: Representative immunoblotting images. Expression of IL-20R was assessed by qRT-PCR and expressed and ΔCt calculated relative to 18S. The ratio of IL-20Rα/β was calculated as a fold change of α-β within each sex. Activation of STAT3 was assessed by immunoblotting of phospho STAT3/total STAT3. Both male and female mice were run on the same gel, and non-essential lanes were cropped for generation of the representative image. Data are expressed as mean ± SEM; n = 4-7 mice per group. STAT3: Signal transducer and activator of transcription 3; DTG: Double transgenic.