Tenascin C upregulates interleukin-6 expression in human cardiac myofibroblasts via toll-like receptor 4

Figure 5 Fibrinogen-like globe domain of Tenascin C up-regulates interleukin-6 mRNA expression in human cardiac myofibroblasts via toll-like receptor 4. A and B: Effect of 1 μmol/L (24 h) recombinant protein, corresponding to either the FBG domain or the fibronectin type III domain (TNIII 5-7) of TNC, on IL-6 mRNA (A) and MMP3 mRNA (B) in human CMF (n = 3-5 donors). Data expressed as mean ± SEM. ^bP < 0.01 vs vehicle (ANOVA post-hoc); C: Effect of 1 h pre-incubation in TLR4 neutralising antisera (25 μg/mL) alone on IL-6 mRNA expression and on FBG (1 μmol/L, 24 h) stimulated IL-6 mRNA expression in CMF (n = 3 donors). Data expressed as mean ± SEM. ^bP < 0.01 vs vehicle (ANOVA post-hoc). FBG: Stimulation with FBG recombinant alone; TLR4-Ab: Pre-incubation with TLR4 neutralising antisera; FBG/TLR4-Ab: Stimulation with FBG following pre-incubation with TLR4 neutralising antisera. FBG: Fibrinogen-like globe; TNC: Tenascin C; IL: Interleukin; CMF: Cardiac myofibroblasts.