Interaction of hyperlipidemia and reactive oxygen species: Insights from the lipid-raft platform

Figure 2 Immunohistochemistry of actin and visualization of vesicle structures after free cholesterol loading and atorvastatin pretreatment in cultured human aortic endothelial cells. The cells were loaded by cholesterol-saturated methyl-b-cyclodextrin (Chol/MBCD) with and without atorvastatin (10 μmol/L) pretreatment. Atorvastatin (Pfizer, New York, NY) pretreatment (C) significantly suppressed formation of vesicles induced by free cholesterol loading, as shown by oil red O as compared with Chol/MBCD loading alone (B); A: Control.