Diagnostic and prognostic value of circulating microRNAs in heart failure with preserved and reduced ejection fraction

Table 2 microRNAs in the diagnosis and differentiation of heart failure with preserved ejection fraction and heart failure with reduced ejection fraction

miRNA	Study type	Bio-material	Groups/size	Detection method	Effect	Value as biomarker	Ref.
miR-211	Experimental	Tissue	Mice - HFpEF vs HC	qRT-PCR	Up-regulated in HFpEF	Diagnostic	[67]
miR-30c	Clinical	Serum	HFpEF n = 90	qRT-PCR	AUC analyses	Differentiating	[122]
			HFrEF n = 90			HFrEF from HFpEF
			HC n = 90
miR-125a-5p	Clinical	Plasma	HFpEF n = 30	qRT-PCR	Up-regulated in HFrEF,	Diagnostic in HFrEF +	[115]
			HFrEF n = 30		unchanged in HFpEF	differentiating HFrEF
			HC n = 28			from HFpEF
miR-146a	Clinical	Serum	HFpEF n = 90	qRT-PCR	AUC analyses	Differentiating HFrEF	[122]
			HFrEF n = 90			from HFpEF
			HC n = 90
miR-183-3p1	Clinical	Plasma	HFpEF n = 30	qRT-PCR	Down-regulated in	Diagnostic	[115]
			HFrEF n = 30		HFrEF and HFpEF
			HC n = 28
miR-190a*	Clinical	Plasma	HFpEF n = 30	qRT-PCR	Down-regulated in	Diagnostic in HFpEF +	[115]
			HFrEF n = 30		HFpEF, unchanged in	differentiating
			HC n = 28		HFrEF	HFrEF from HFpEF
miR-193b-3p1	clinical	Plasma	HFpEF n = 30	qRT-PCR	Down-regulated in	Diagnostic	[115]
			HFrEF n = 30		HFrEF and HFpEF
			HC n = 28
miR-193b-5p1	Clinical	Plasma	HFpEF n = 30	qRT-PCR	Down-regulated in HFpEF	Diagnostic	[115]
			HFrEF n = 30
			HC n = 28
miR-211-5p1	Clinical	Plasma	HFpEF n = 30	qRT-PCR	Down-regulated in HFrEF	Diagnostic	[115]
			HFrEF n = 30
			HC n = 28
miR-221	Clinical	Serum	HFpEF n = 90	qRT-PCR	AUC analyses	Differentiating	[122]
			HFrEF n = 90			HFrEF from HFpEF
			HC n = 90
miR-328	Clinical	Serum	HFpEF n = 90	qRT-PCR	AUC analyses	Differentiating	[122]
			HFrEF n = 90			HFrEF from HFpEF
			HC n = 90
miR-375	Clinical	Serum	HFpEF n = 90	qRT-PCR	AUC analyses	Differentiating	[122]
			HFrEF n = 90			HFrEF from HFpEF
			HC n = 90
miR-454	Clinical	Plasma	HFpEF n = 8	qRT-PCR	Down-regulated in HFpEF	Diagnostic	[121]
			Stable DCM n = 10
			Decompensated DCM n = 13
			HC n = 8
miR-4941	Clinical	Plasma	HFpEF n = 30	qRT-PCR	Down-regulated in HFrEF	Diagnostic	[115]
			HFrEF n = 30
			HC n = 28
miR-500	Clinical	Plasma	HFpEF n = 8	qRT-PCR	Down-regulated in HFpEF	Diagnostic	[121]
			Stable DCM n = 10
			Decompensated DCM n = 13
			HC n = 8
miR-545-5p	Clinical	Plasma	HFpEF n = 30	qRT-PCR	Up-regulated in HFpEF	Diagnostic	[115]
			HFrEF n = 30
			HC n = 28
miR-550a-5p	Clinical	Plasma	HFpEF n = 30	qRT-PCR	Up-regulated in HFrEF	Differentiating	[115]
			HFrEF n = 30		Down-regulation in HFpEF	HFrEF from HFpEF
miR-638	Clinical	Plasma	HFpEF n = 30	qRT-PCR	Down-regulated in HFrEF,	Diagnostic in HFrEF +	[115]
			HFrEF n = 30		unchanged in HFpEF	differentiating HFrEF
			HC n = 28			from HFpEF
miR-671-5p1	Clinical	Plasma	HFpEF n = 30	qRT-PCR	Up-regulated in HFrEF	Diagnostic	[115]
			HFrEF n = 30
			HC n = 28
miR-12331	Clinical	Plasma	HFpEF n = 30	qRT-PCR	Up-regulated in HFpEF	Diagnostic	[115]
			HFrEF n = 30
			HC n = 28
miR-1246	Clinical	Plasma	HFpEF n = 8	qRT-PCR	Down-regulated in HFpEF	Diagnostic	[121]
			Stable DCM n = 10
			Decompensated DCM n = 13
			HC n = 8

¹See also Table 1. HC: Healthy control; miRNA: microRNAs; DCM: Dilated cardiomyopathy; HFpEF: Heart failure with preserved ejection fraction; HFrEF: Heart failure with reduced ejection fraction; qRT-PCR: Quantitative real time polymerase chain reaction; AUC: Area under the operating receiver curve.