Translational research of adult stem cell therapy

Figure 1 Toll-like receptor 3-mesenchymal stem cells enhance to secrete paracrine factors. RNA mimetic polyinosinic-polycytidylic acid [poly(I:C)] stimulated TLR3 system on MSCs. TLR3-MSCs secreted a variety of paracrine factors. RT-PCR detected significant upregulation of SDF1, VEGF and IL6 while inflammation related cytokines (IL-1α, TNFα) were downregulated. Injection of TLR3-MSCs in cardiomyopathy model improved cardiac function more than standard MSCs in association with increasing myocyte proliferation, reducing fibrosis and myocyte apoptosis. TLR3: Toll-like receptor 3; MSCs: Mesenchymal stem cells; SDF1: Stromal cell-derived factor-1; VEGF: Vascular endothelial growth factor; IL: Interleukin; TNF: Tumor necrosis factor.