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World J Cardiol. Sep 26, 2014; 6(9): 968-984
Published online Sep 26, 2014. doi: 10.4330/wjc.v6.i9.968
Published online Sep 26, 2014. doi: 10.4330/wjc.v6.i9.968
Figure 5 Hypertension-induced Angiotensin II up-regulated monocyte chemoattractant protein-1 and monocyte chemoattractant protein-1 induced protein expression through AT1R activation in retinal pigmented epithelium-choroid[140].
C57BL 6 mice were treated with saline (1), Ang II (2), and Ang II in combination with candesartan (10 mg/kg per day) (3). Blood pressure was recorded before and after treatment. After 30 d of treatment, animals were sacrificed and eyes enucleated and collected for microdisection of retinal pigmented epithelium-choroid. Monocyte chemoattractant protein-1 (MCP-1) and MCP-1 induced protein (MCPIP) proteins were analyzed by real-time PCR and Western blot. MCP-1 and MCPIP mRNA expression by real-time PCR (A and D), protein expression by Western blot (B and E), and MCP-1 protein secretion by ELISA (C). GAPDH was used as control. Data are expressed as mean ± SE (n = 3). aP < 0.05, bP < 0.01 vs control; cP < 0.05, dP < 0.01 vs Ang II-treated animals. CD: Candesartan. Ang: Angiotensin; PCR: Polymerase chain reaction; GAPDH: Glyceraldegyde 3-phosphate dehydrogenase; ELISA: Enzyme-linked immuno assay.
- Citation: Marin Garcia PJ, Marin-Castaño ME. Angiotensin II-related hypertension and eye diseases. World J Cardiol 2014; 6(9): 968-984
- URL: https://www.wjgnet.com/1949-8462/full/v6/i9/968.htm
- DOI: https://dx.doi.org/10.4330/wjc.v6.i9.968