Molecular phenotypes of human parvovirus B19 in patients with myocarditis

Table 2 Primer sequences

No	Primer name	Sequences (5’ to 3’)	Position (numbering according M13178)	1st, 2nd (RT/RFLP)-PCR
1	PVB1	GCTAACTCTGTAACTTGTAC	3221-3240	Sense B19V-VP2-PCR (2nd PCR)
2	PVB2	AAATATCTCCATGGGGTTGAG	3373-3393	As B19V-VP2-PCR (2nd PCR)
3	PVB3	AGCATGTGGAGTGAGGGGGC	3191-3210	Sense B19V-VP2-PCR (1st PCR)
4	PVB4	AAAGCATCAGGAGCTATACTTCC	3458-3480	As B19V-VP2-PCR (1st PCR)
5	NS-25	AAATGCGTGGAAGTGTAGCT	1628-1647	Sense B19V-NS1-PCR (RT/1st PCR)
6	NS-27	ATGCGTGGAAGTGTAGCTGT	1630-1649	As B19V-NS1-PCR (2nd PCR)
7	NS-30	CCAACTAACAGTTCACGAAAC	2172-2192	Sense B19V-NS1-PCR (RT/1st PCR)
8	NS-32	TAACAGTTCACGAAACTGGTC	2168-2187	As B19V-NS1-PCR (2nd PCR)
9	NS-38	ATTCCACAAATTGCTGATACAC	2498-2519	As RFLP-PCR (1st PCR)
10	NS-40	AATTGCTGATACACAGCTTTAG	2490-2511	As RFLP-PCR (2nd PCR)
11	G2170	CAGTTTCGTGAACTGTTAGT	2170-2189	Sense RFLP-PCR (1st PCR)
12	G2176	CGTGAACTGTTAGTTGGGGTTGA	2176-2198	Sense RFLP-PCR (2nd PCR)

As: Antisense; RFLP: Restriction fragment length polymorphism; RT-PCR: Reverse transcriptase-polymerase chain reaction.