GRK5 is an essential co-repressor of the cardiac mineralocorticoid receptor and is selectively induced by finerenone

Figure 4 Comparison of the anti-fibrotic efficacies of finerenone vs eplerenone in aldosterone-treated cardiomyocytes in the presence or absence of G protein-coupled receptor-kinase 5. mRNA levels of PAI-1 (A) and fibronectin (B), in response to a 2-h-long treatment of 100 nmol/L aldosterone in the presence of either 10 mmol/L eplerenone (Epl) or 10 mmol/L finerenone in control (mock, empty vector CRISPR lentivirus-infected) or in G protein-coupled receptor-kinase (GRK)-5-KO (rat GRK5-targeting CRISPR lentivirus-infected) cells. 18S rRNA levels were used for normalization of the results. ^aP < 0.05, vs Epl; n = 3 independent measurements per cell clone/treatment performed in triplicate. Epl: Eplerenone; Fin: Finerenone.