GRK5 is an essential co-repressor of the cardiac mineralocorticoid receptor and is selectively induced by finerenone

Figure 2 G protein-coupled receptor-kinase 5 inhibits the cardiac mineralocorticoid receptor in response to finerenone but not to eplerenone. Transcriptional activity of the mineralocorticoid receptor (MR) in response to either 10 mmol/L eplerenone or 10 mmol/L finerenone in H9c2 cardiomyocytes overexpressing G protein-coupled receptor-kinase (GRK)-5 or having GRK5 genetically deleted via CRISPR (GRK5-KO). Neither agent was able to suppress MR basal transcriptional activity in GRK5-KO cells. ^aP < 0.05, vs eplerenone; n = 5 independent measurements per cell clone/treatment performed in triplicate. CNCV: CRISPR negative control virus-infected control cells; GRK5-OE: G protein-coupled receptor-kinase 5-overexpressing.