NBCe1 Na+-HCO3- cotransporter ablation causes reduced apoptosis following cardiac ischemia-reperfusion injury in vivo

Figure 2 Polymerase chain reaction genotyping and quantitative reverse transcriptase-polymerase chain reaction of NBCe1 mRNA. A: PCR analysis of DNA from tail biopsies, using primers flanking the proximal LoxP site, showing bands for the alleles in Slc4a4^+/+, Slc4a4^flx/+ and Slc4a4^flx/flx mice. The larger size of the band for the floxed allele is due to the presence of the LoxP site; B: Quantitative RT-PCR analysis of cDNA prepared from whole heart RNA (n = 6 for each genotype) showing a reduction of Slc4a4 mRNA in hearts of KO (Slc4a4^flx/flx(Cre)) relative to WT (Slc4a4^flx/flx without Cre) mice. PCR: Polymerase chain reaction.