NBCe1 Na+-HCO3- cotransporter ablation causes reduced apoptosis following cardiac ischemia-reperfusion injury in vivo

Figure 1 Targeting strategy for generation of Slc4a4 cardiac myocyte conditional KO mice. The targeting construct contained the neomycin resistance gene (neo) to allow selection of ES cells after homologous recombination. The neo gene was flanked with flippase recognition target (FRT) sites, which allowed removal of neo when mice carrying the targeted allele were bred with transgenic mice expressing Flip recombinase. After confirming the deletion of neo, mice carrying the floxed allele were bred with mice expressing Cre recombinase controlled by the β-MHC promoter, which mediates the deletion of exon 12 of Slc4a4 in cardiac myocytes.